OVERFRAG: an overlapping DNA fragments generator for molecular cloning and synthetic biology
Coding Sequence Without Introns:
ATG CAT GTA TTA TCT ACC GCA GTT CTG CTT GGA AGC GTT GCG GTC CAA AAG GTT CTA GGT AGA CCT GGG TCA TCA GGT TTA AGT GAC GTG ACT AAA AGG AGC GTC GAT GAT TTC ATC TCA ACC GAG ACC CCA ATA GCA CTA AAT AAT CTA CTT TGT AAC GTT GGT CCA GAT GGT TGC AGG GCG TTC GGC ACT AGC GCT GGG GCC GTC ATT GCT TCT CCC TCT ACT ATC GAC CCA GAT TAT TAT TAC ATG TGG ACT CGT GAT TCC GCG TTG GTG TTC AAG AAT TTA ATC GAT CGT TTC ACG GAA ACG TAT GAT GCA GGC CTA CAA CGT AGA ATA GAA CAG TAT ATA ACT GCC CAA GTC ACC TTG CAG GGT TTG TCT AAT CCC TCC GGG AGC CTG GCA GAT GGA TCC GGT TTA TG CAG GGT TTG TCT AAT CCC TCC GGG AGC CTG GCA GAT GGA TCC GGT TTAGGT GAG CCT AAG TTT GAA TTA ACT CTT AAA CCT TTT ACT GGT AAC TGG GGT CGT CCC CAA CGT GAT GGT CCT GCT CTG AGA GCG ATT GCC CTG ATT GGT TAT TCT AAA TGG TTG ATA AAT AAC AAC TAT CAG TCT ACT GTG TCC AAC GTA ATC TGG CCT ATC GTG AGG AAT GAC TTA AAT TAC GTT GCA CAA TAT TGG AAC CAG ACT GGT TTC GAT TTG TGG GAG GAA GTA AAT GGA TCC TCC TTC TTT ACT GTA GCT AAT CAA CAT CGT GCC CTT GTT GAA GGT GCT ACT TTA GCC GCA ACT TTG GGT CAA TCT GGT AGT GCT TAT AGC AGC GTT GCG CCA CAA GTA CTA TGC TTC TTA CAG AGA TTC TGG GTT TCA TCC GGG GGA TAT GTA GAT TCA AAT ATA AAC ACCAAC GAA GGA AGA ACC GGT AAG GAT GTT AAT TCC GTA CTA ACT TCT ATC CAT ACG TTT GAT CCT AAT CTG GGG TGC GAT GCG GGT ACC TTT CAG CCA TGC TCA GAT AAA GCA TTA TCC AAT CTT AAG GTG GTT GTT GAC TCA TTC CGT TCA ATC TAC GGT GTT AAT AAA GGA ATC CCT GCT GGC GCT GCT GTA GCT ATT GGT AGA TAT GCT GAA GAC GTG TAC TAC AAC GGA AAT CCA TGG TAC TTG GCT ACT TTT GCT GCT GCT GAA CAA TTA TAT GAC GCA ATT TAC GTG TGG AAA AAA ACC GGC TCA ATC ACC GTT ACC GCT ACA TCT TTA GCT TTT TTC CAA GAG TTA GTT CCA GGC GTA ACA GCA GGC ACT TAC AGT TCC TCT TCT TCT ACA TTC ACA AAT ATT ATC AAT GCT GTG TCC ACG TAT GCT GAT GGT TTT TTA TCT GAA GCT GCC AAG TAC GTA CCG GCG GAC GGT TCT CTG GCG GAA CAA TTC GAT AGA AAC TCT GGA ACC CCC TTA AGC GCG CTA CAT TTA ACC TGG TCT TAT GCC TCA TTC TTG ACC GCA ACA GCA AGA AGA GCT GGG ATC GTG CCG CCA AGT TGG GCC AAC TCC TCT GCT TCT ACT ATT CCC AGT ACT TGC TCA GGT GCA AGT GTG GTA GGT AGT TAT TCA AGG CCT ACT GCT ACA TCC TTC CCA CCC TCT CAA ACA CCT AAG CCG GGA GTG CCT AGC GGC ACC CCA TAC ACC CCC TTG CCA TGT GCT ACT CCC ACA TCA GTT GCT GTT ACA TTT CAT GAG CTA GTC TCA ACT CAG TTC GGA CAG ACT GTA AAG GTC GCA GGC AAC GCA GCC GCT TTG GGT AAT TGG AGT ACT TCC GCT GCC GTG GCT TTA GAT GCT GTT AAT TAC GCG GAT AAC CAC CCA CTA TGG ATT GGT ACC GTG AAT CTT GAG GCA GGT GAT GTT GTA GAG TAT AAG TAC ATA AAT GTT GGT CAG GAC GGT TCT GTT ACT TGG GAG TCT GAT CCT AAT CAC ACA TAT ACA GTT CCT GCC GTC GCC TGT GTT ACG CAG GTG GTT AAA GAG GAC ACT TGG CAA AGTCTG GTG CCA CGC GGT AGT TCC GCT CAT CAC CAC CAT CAT CAC TAA
Promoter Sequence:
TTACCCATAAGGTTGTTTGTGACGGCGTCGTACAAGAGAACGTGGGAACTTTTTAGGCTCACCAAAAAAGAAAGAAAAAATACGAGTTGCTGACAGAAGCCTCAAGAAAAAAAAAATTCTTCTTCGACTATGCTGGAGGCAGAGATGATCGAGCCGGTAGTTAACTATATATAGCTAAATTGGTTCCATCACCTTCTTTTCTGGTGTCGCTCCTTCTAGTGCTATTTCTGGCTTTTCCTATTTTTTTTTTTCCATTTTTCTTTCTCTCTTTCTAATATATAAATTCTCTTGCATTTTCTATTTTTCTCTCTATCTATTCTACTTGTTTATTCCCTTCAAGGTTTTTTTTTAAGGAGTACTTGTTTTTAGAATATACGGTCAACGAACTATAATTAACTAAAC
Terminator Sequence:
ACAGGCCCCTTTTCCTTTGTCGATATCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCTCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCC
Maximum Fragment Size:
Overlap Size:
Minimum GC content (from 0 to 90):
Maximum GC content (from 10 to 100):
Maximum extra fragments:
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